Influence of pain-relieving therapies on inflammation and the expression of proinflammatory neuropeptides after dental bleaching treatment
da Silva Livia Maria Alves Valentim, Cintra Luciano Tavares Angelo, de Oliveira Gallinari Marjorie, Benetti Francine, Rahal Vanessa, Ervolino Edilson, de Alcantara Sibele, Briso Andre Luiz Fraga,
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( da Silva Livia Maria Alves Valentim ) - Sao Paulo State University School of Dentistry Department of Restorative Dentistry
( Cintra Luciano Tavares Angelo ) - Sao Paulo State University School of Dentistry Department of Restorative Dentistry
( de Oliveira Gallinari Marjorie ) - Sao Paulo State University School of Dentistry Department of Restorative Dentistry
( Benetti Francine ) - Universidade Federal de Minas Gerais Department of Restorative Dentistry
( Rahal Vanessa ) - Sao Paulo State University School of Dentistry Department of Restorative Dentistry
( Ervolino Edilson ) - Sao Paulo State University School of Dentistry Department of Basic Sciences
( de Alcantara Sibele ) - Sao Paulo State University School of Dentistry Department of Restorative Dentistry
( Briso Andre Luiz Fraga ) - Sao Paulo State University School of Dentistry Department of Restorative Dentistry
Abstract
Objectives: To minimize the tooth sensitivity caused by in-office bleaching, many dentists use non-steroidal anti-inflammatory drugs and topical desensitizing gels containing potassium nitrate and sodium fluoride. This study aimed to evaluate the influence of these substances on inflammation and the expression of substance P and calcitonin gene-related peptide in pulp nerve fibers.
Materials and Methods: Seventy-two rats were divided into 6 groups as follows: GI, control; GII, only dental bleaching; GIII, only ibuprofen; GIV, ibuprofen administered 30 minutes before and after the bleaching treatment and every 12 hours until the analysis; GV, only topical application of a desensitizing agent; and GVI, topical application of a desensitizing agent before dental bleaching. Placebo gel was applied to the upper left jaw and the bleaching agent was applied to the upper right jaw in all groups. Subsequently, the groups were divided into 3 subgroups based on the time of analysis: 0, 24, and 48 hours after bleaching (n = 8). The rats were euthanized and the maxillae were processed and evaluated by histopathological and immunohistochemical analyses. The data were analyzed using the Kruskal-Wallis test, followed by the Dunn test (p < 0.05).
Results: In the bleaching groups, the inflammatory process and expression of neuropeptides decreased over time. The animals in which a desensitizing agent was applied showed better results within 24 hours.
Conclusions: The use of a desensitizing agent had positive effects on inflammation and pain-related neuropeptide expression, minimizing the painful effects of dental bleaching treatment.
키워드
Tooth bleaching; Hydrogen peroxide; Ibuprofen; Laboratory research; Pain; Reactive oxygen species
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